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KMID : 0377619720230020157
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Korean Jungang Medical Journal
1972 Volume.23 No. 2 p.157 ~ p.162
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Effects of Glycerin on Preservation of Vibrio and Salmonella
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Abstract
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Several media containing glycerin at 30 per cent was reported as transport media of specimens containing Vibrio and Salmonella. However, as the results with these media were not always satisfactory for the transport of specimens, we carried out some experiments to evaluate the role of glycerin for thepreservation of Vibrio and Salmonella.
Vibrio parahaemolyticzcs and V. cholerae were easily and rapidly sterilized in the presence of 30 per cent glycerin in phosphate buffer containing saline, and -the already reported transport media containing 30 per cent glycerin would not be used for the transport of feces and other specimens containing Vibrio. The number ´of Salmonella in phosphate buffer containing 30 per cent glycerin. decreased to about a half of original numbers after 24 hours at 20°C, and the numbers of Escherichia coli and other microorganisms in feces also decreased. to the same degree. Therefore, the relative number of Salmonella over enteric flora in feces is not decreased in the medium, and the media containing 30 per cent glycerin would be used for the transport of Salmonella-containing specimens. The toxic effect of 15 per cent glycerin on Vibrio was weak, and the organism propagated slightly in the presence of 15 per cent glycerin at 20°C. However, the increase in number of E. coli and other enteric flora was, more marked than Vibrio and Salmonella, resulting in the decrease of relative concentration of Vibrio and Salmonella, and phosphate buffer containing 15 per cent glycerin is not suitable for the preservation or transport of Vibrio or Salmonella-containing specimens. The effect of temperature on the role of glycerin for the preservation of Vibrio and Salmonella was studied, and it was found that the sterilizing effect of 30 per cent glycerin was a little more marked at 30°C.
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